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Confirmation of Staphylococcal toxins by PCR

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Reference Details


Confirmation of Staphylococcal toxins by PCR.

Test Category:
Molecular Detection
Staphylococcus aureus
Illnesses and Diseases:
  • Necrotizing pneumonia
  • Skin and soft tissue infections

Pure culture.

Collection Method:


Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium. Culture vessel should be leak-proof or sealed appropriately.


Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:


Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.


Please consult lab prior to requesting test.

Methods and Interpretation of Results:

PCR is used to confirm presence or absence of staphylococcal toxins.  Toxin genes include enterotoxins A (sea), B (seb), C (sec), D (sed), E (see), G (seg), H (seh) and I (sei); exfoliative toxin A (eta) and B (etb) and Toxic Shock Syndrome Toxin 1 (tst). Sample compared to known Standards.

Turnaround Time:

6 calendar days.

Phone #: (204) 789-5000
Fax: (204) 789-5020
  1. Mehrotra M., G. Wang and W.M. Johnson. 2000. Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1 and Methicillin Resistance. J. Clin. Micro. 38(3):1032-1035.
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