Confirmation of Staphylococcal toxins by PCR

Confirmation of Staphylococcal toxins by PCR.

• Necrotizing pneumonia
• Skin and soft tissue infections

Pure culture.

N/A

Send cultures as growth on culture medium or in transport medium. Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

N/A

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Please consult lab prior to requesting test.

PCR is used to confirm presence or absence of staphylococcal toxins.  Toxin genes include enterotoxins A (sea), B (seb), C (sec), D (sed), E (see), G (seg), H (seh) and I (sei); exfoliative toxin A (eta) and B (etb) and Toxic Shock Syndrome Toxin 1 (tst). Sample compared to known Standards.

6 calendar days.

1. Mehrotra M., G. Wang and W.M. Johnson. 2000. Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1 and Methicillin Resistance. J. Clin. Micro. 38(3):1032-1035.

Pathogen Safety Data Sheets: Infectious Substances – Staphylococcus aureus

Fact Sheet - Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA)