Ribotyping

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Reference Details

Description:

Ribotyping of Clostridium difficile.

Test Category:
Genetic Fingerprinting
Pathogen:
Clostridium difficile
Illnesses and Diseases:
  • Pseudomembranous colitis
Specimen:

Pure culture or 1.0mL frozen stool sample.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

N/A

Methods and Interpretation of Results:

Ribotyping is a sequenced based method that generates ribotypes that are compared to an international database.

Turnaround Time:

10 Calendar days.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Indra A., S. Huhulescu, M. Schneeweis, P. Hasenberger, S. Kernbichler, A. Fiedler, G. Wewalka, F. Allerberger, and E. J. Kuijper. 2008. Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol 57:1377-1382.
  2. Fawley WN, et al. 2015. Development and validation of an internationally-standardized, high-resolution capillary gel-based electrophoresis PCR-ribotyping protocol for Clostridium difficile. PLoS One, 10(2):e0118150.doi: 10.1371/journal.pone.0118150.eCollection 2015.