Ribotyping

Ribotyping of Clostridium difficile.

• Pseudomembranous colitis

Pure culture or 1.0mL frozen stool sample.

N/A

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

N/A

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

N/A

Ribotyping is a sequenced based method that generates ribotypes that are compared to an international database.

10 Calendar days.

1. Indra A., S. Huhulescu, M. Schneeweis, P. Hasenberger, S. Kernbichler, A. Fiedler, G. Wewalka, F. Allerberger, and E. J. Kuijper. 2008. Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol 57:1377-1382.
2. Fawley WN, et al. 2015. Development and validation of an internationally-standardized, high-resolution capillary gel-based electrophoresis PCR-ribotyping protocol for Clostridium difficile. PLoS One, 10(2):e0118150.doi: 10.1371/journal.pone.0118150.eCollection 2015.

Fact Sheet – Clostridium difficile (C. difficile)

Pathogen Safety Data Sheets: Infectious Substances – Clostridium difficile

Case Definitions for Communicable Diseases under National Surveillance - 2009 - Clostridium difficile associated diarrhea

C. difficile (Clostridium difficile)