Ribotyping

Ribotyping of Clostridioides difficile.

• Pseudomembranous colitis

Pure culture or 1.0mL frozen stool sample.

N/A

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice. Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

N/A

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

N/A

Ribotyping is a sequenced based method that generates ribotypes that are compared to an international database.

10 Calendar days.

1. Indra A., S. Huhulescu, M. Schneeweis, P. Hasenberger, S. Kernbichler, A. Fiedler, G. Wewalka, F. Allerberger, and E. J. Kuijper. 2008. Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol 57:1377-1382.
2. Fawley WN, et al. 2015. Development and validation of an internationally-standardized, high-resolution capillary gel-based electrophoresis PCR-ribotyping protocol for Clostridium difficile. PLoS One, 10(2):e0118150.doi: 10.1371/journal.pone.0118150.eCollection 2015.

Fact Sheet – Clostridioides difficile (C. difficile) - formerly known as Clostridium difficile

Pathogen Safety Data Sheets: Infectious Substances – Clostridioides difficile - formerly known as Clostridium difficile

National Case Definition : Clostridioides difficile associated diarrhea - formerly known as Clostridium difficile

C. difficile (Clostridioides difficile) - formerly known as Clostridium difficile