Language Selection

Français

Ribotyping

<<Return to Search Results

Requisition Forms

Reference Details

Description:

Ribotyping of Clostridioides difficile.

Test Category:
Molecular Typing
Pathogen:
Clostridioides difficile
Illnesses and Diseases:
  • Pseudomembranous colitis
Specimen:

Pure culture or 1.0mL frozen stool sample.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice. Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

N/A

Methods and Interpretation of Results:

Ribotyping is a sequenced based method that generates ribotypes that are compared to an international database.

Turnaround Time:

10 Calendar days.

Contact:
Phone #: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Indra A., S. Huhulescu, M. Schneeweis, P. Hasenberger, S. Kernbichler, A. Fiedler, G. Wewalka, F. Allerberger, and E. J. Kuijper. 2008. Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol 57:1377-1382.
  2. Fawley WN, et al. 2015. Development and validation of an internationally-standardized, high-resolution capillary gel-based electrophoresis PCR-ribotyping protocol for Clostridium difficile. PLoS One, 10(2):e0118150.doi: 10.1371/journal.pone.0118150.eCollection 2015.