Confirmation and toxin test by PCR

<<Return to Laboratory

Requisition Forms

Reference Details

Description:

Clostridium difficile confirmation and toxin test by PCR.

Test Category:
Molecular Detection
Pathogen:
Clostridium difficile
Illnesses and Diseases:
  • Pseudomembranous colitis
Specimen:

Pure culture or 1.0mL frozen stool sample.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

N/A

Methods and Interpretation of Results:

PCR is used to confirm presence or absence of C. difficile and its toxins. Results are compared to known Standards.

Turnaround Time:

10 Calendar days.

Contact:
Phone: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Lemee, L., A. Dhalluin, S. Testelin, M.-A. Mattrat, K. Maillard, J.-F. Lemeland, and J.L. Pons. 2004. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB genes for toxigenic culture of Clostridium difficile. J. Clin.  Microbiol. 42:5710-5714.
  2. Spigaglia, P., and P. Mastrantonio. 2004. Comparative analysis of Clostridium   difficile clinical isolates belonging to different genetic lineages and time periods. J. Med. Microbiol. 53:1129-1136.