Confirmation and toxin test by PCR<<Return to Laboratory
Clostridium difficile confirmation and toxin test by PCR.
- Pseudomembranous colitis
Pure culture or 1.0mL frozen stool sample.
Send cultures as growth on culture medium or in transport medium. Send frozen stool samples on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
For additional guidance on the transport of infectious substances in other languages, please click on the link below.
Completed Antimicrobial Resistance and Nosocomial Infections Requisition.
PCR is used to confirm presence or absence of C. difficile and its toxins. Results are compared to known Standards.
10 Calendar days.
- Lemee, L., A. Dhalluin, S. Testelin, M.-A. Mattrat, K. Maillard, J.-F. Lemeland, and J.L. Pons. 2004. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42:5710-5714.
- Spigaglia, P., and P. Mastrantonio. 2004. Comparative analysis of Clostridium difficile clinical isolates belonging to different genetic lineages and time periods. J. Med. Microbiol. 53:1129-1136.