Antimicrobial Susceptibility Testing

Antimicrobial susceptibility testing of Clostridioides difficile.

Pure culture or 1.0mL frozen stool sample.

N/A

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice. Culture vessel should be leak-proof or sealed appropriately.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

N/A

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

N/A

Susceptibility testing is performed at the discretion of the lab by Etest or broth microdilution. Results are interpreted according to breakpoints published by CLSI, FDA, or EuCAST.

12 Calendar days, however if growth is slow 19 calendar days.

1. CLSI. Performance Standards for Antimicrobial Susceptibility Testing, 33rd informational supplement, M100-S27. 2023. Wayne, PA: Clinical and Laboratory Standards Institute.
2. The European Committee on Antimicrobial Susceptibility Testing (EuCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 13, 2023. http//www.eucast.org