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Antimicrobial Susceptibility Testing

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Requisition Forms

Reference Details

Description:

Antimicrobial susceptibility testing of Clostridioides difficile.

Test Category:
Susceptibility Testing
Pathogen:
Clostridioides difficile
Illnesses and Diseases:
  • Pseudomembranous colitis
Specimen:

Pure culture or 1.0mL frozen stool sample.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send cultures as growth on culture medium or in transport medium.  Send frozen stool samples on dry ice. Culture vessel should be leak-proof or sealed appropriately.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

N/A

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

N/A

Methods and Interpretation of Results:

Susceptibility testing is performed at the discretion of the lab by Etest, broth microdilution or Vitek2. Results are interpreted according to breakpoints published by CLSI, FDA or EuCAST.

Turnaround Time:

12 Calendar days, however if growth is slow 19 calendar days.

Contact:
Phone #: (204) 789-5000
Fax: (204) 789-5020
References:
  1. CLSI. Performance Standards for Antimicrobial Susceptibility Testing, 33rd informational supplement, M100-S27. 2023. Wayne, PA: Clinical and Laboratory Standards Institute.
  2. The European Committee on Antimicrobial Susceptibility Testing (EuCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 13, 2023. http//www.eucast.org