Detection of antibodies against Tick-borne encephalitis virus by ELISA

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Reference Details

Description:

Detection of antibodies against tick-borne encephalitis virus by enzyme linked immunosorbent assay (ELISA).

Test Category:
Serology
Pathogen:
Tick-borne encephalitis virus
Illnesses and Diseases:
  • Tick-borne encephalitis
Specimen:

ELISA, PCR and isolation: whole blood, sera or CSF.

Collection Method:

Collect serum using SST tubes and whole blood in EDTA tubes, other samples can be in sterile 1.5-2.0 mL tubes.

Specimen Processing, Storage and Shipping:

Store samples in refrigerator or frozen until being shipped for testing.  Ship frozen specimens on dry ice and refrigerated specimens on wet ice. Avoid freezing whole blood samples.

Transportation of Dangerous Goods:

Store samples in refrigerator or frozen until being shipped for testing. Ship frozen specimens on dry ice and refrigerated samples on wet ice. Shipping of samples suspected of containing Risk Group 4 pathogens shall be done by a TDG certified individual in accordance with TDG regulations. If applicable, activate Emergency Response Assistance Plan (ERAP) 24 hours prior to shipping. To activate an ERAP, please click on the link below to access the transport flowchart for Risk Group 4 pathogens or call 204-999-7996 (available 24/7) for assistance.

Transport Flowchart for Risk Group 4 Pathogens

Patient Criteria:

Symptomatic and travel to TBE virus endemic areas or contact to travellers from these areas.

Accompanying Documentation:

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.

Methods and Interpretation of Results:

For diagnosis of TBE infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of TBE virus RNA by PCR,  (ii) isolation of TBE virus and (iii)TBE specific IgM or rising titers of IgG antibodies.

Turnaround Time:

2 calendar days.

Contact:
Phone: (204) 789-6019 or (204) 789-7032
Fax: (204) 789-2140
References:
Fact Sheets: