Antimicrobial Susceptibility Testing On Selected Aerobes, Facultative Anaerobes, and Strict Anaerobes<<Return to Laboratory
Antimicrobial Susceptibility Testing (AST) is done by the broth microdilution method as described by the Clinical and Laboratory Standards Institute (CLSI) guidelines.
- Bacterial endocarditis
- Urinary tract infection
Pure cultures of isolates recovered from normally sterile sites and serious wound infections.
Slants or plates of any suitable media and swabs in transport media are all acceptable.
Store samples at room temperature until shipped for testing. Ship at room temperature.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Clinical illness with symptoms suggestive of bacterial infection.
Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient and strain history must be included. Other non-mycobacterial, non-enteric, Gram positive/negative bacteria which are not described by a CLSI guideline may be tested upon request and at the discretion of the Special Bacteriology Laboratory. Testing does not include any Risk Group 3 organisms as well as Capnocyphaga spp., other capnophiles, and Streptobacillus moniliformis. Please note that duplicate bacterial isolates that have been isolated from the same patient, source, and identified as the same organism by the sender, will be rejected unless additional information is provided to warrant testing. Acceptance and/or rejection of the additional sample(s) is at the discretion of the Special Bacteriology lab. Contact the lab for further information.
Broth microdilution using commercially-available Sensititre® panels. Interpretations are based on the most recent CLSI guidelines. Interpretations for rare or undescribed taxons may not be possible.
16 calendar days. In cases where staff or resources are limited final report may be delayed and status of request will be forwarded in a preliminary report. Poor- or slow-growing isolates may require longer turn around times.
- Clinical and Laboratory Standards Institute. 2021. Performance Standards for Antimicrobial Susceptibility Testing; M100-Ed31. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania.
- Clinical and Laboratory Standards Institute. 2016. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; M45-Ed3. Volume 35, Number 17. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania.
- Clinical and Laboratory Standards Institute. 2018. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard-Ninth Edition. Volume 38. Number 19. M11-Ed9. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania.
- Clinical and Laboratory Standards Institute. 2018. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; M07-Ed11. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania.