Viral Load Quantification

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Requisition Forms

Reference Details

Description:

Determination of Hepatitis E viral load by commercial quantitative Real-Time PCR.

Test Category:
Molecular Quantification
Pathogen:
Hepatitis E virus (HEV)
Illnesses and Diseases:
  • Hepatitis E
Specimen:

Serum or plasma sample.  Minimum volume required for serum or plasma is 1.0 mL.

 

Collection Method:

Collect blood in serum separator tubes (SST) or EDTA tubes.

Specimen Processing, Storage and Shipping:

Store samples frozen until shipped for testing.  Ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Serologically confirmed acute or chronic Hepatitis E virus infection. 

Accompanying Documentation:

Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number.  Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested.  Type of specimen and date collected.  If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Comments:

N/A

Methods and Interpretation of Results:

Commercially available Real Time PCR (RealStar® HEV RT-PCR Kit - Altona Diagnostics, Germany) amplifying all HEV genotypes.

Turnaround Time:

19 calendar days.

 

Contact:
Phone: (204) 789-6061; HCV (204) 789-5063
Fax: (204) 789-2082
References:
  1. Florence Abravanel, Sabine Chapuy-Regaud, Sébastien Lhomme, Martine Dubois, Jean-Marie Peron, Laurent Alric, Lionel Rostaing, Nassim Kamar, Jacques Izopeta,Performance of Two Commercial Assays for Detecting Hepatitis E Virus RNA in Acute or Chronic Infection: 2013, J Clin Microbiol v.51, 1913-16.

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