Viral Load Quantification<<Return to Search Results
Determination of Hepatitis E viral load by commercial quantitative Real-Time PCR.
- Hepatitis E
Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Serologically confirmed acute or chronic Hepatitis E virus infection and/or a positive RNA result from the RT-PCR assay for molecular detection.
Completed Viral Hepatitis and Bloodborne Pathogens requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
Commercially available Real Time PCR (RealStar® HEV RT-PCR Kit - Altona Diagnostics, Germany) amplifying all HEV genotypes.
19 calendar days.
Florence Abravanel, Sabine Chapuy-Regaud, Sébastien Lhomme, Martine Dubois, Jean-Marie Peron, Laurent Alric, Lionel Rostaing, Nassim Kamar, Jacques Izopeta,Performance of Two Commercial Assays for Detecting Hepatitis E Virus RNA in Acute or Chronic Infection: 2013, J Clin Microbiol v.51, 1913-16.