Detection of IgM Antibodies Directed Towards Zika Virus by ELISA

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Requisition Forms

Reference Details

Description:

Serological detection of IgM antibodies directed towards Zika (ZIKV) virus by In-House MAC ELISA.

Test Category:
Serology
Pathogen:
Zika virus
Laboratory:
Illnesses and Diseases:
  • Zika virus infection
Specimen:

Serum. Minimum volume of 250 µL required.

Collection Method:

2 mL screw cap tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected Zika virus infection ORpregnancy status and relevant travel history. See ZIKV submission guidelines.

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, clinical symptoms, and travel history of patient.

Comments:

All ZIKV diagnostic samples are tested by both IgM and IgG ELISA to increase the clinical sensitivity. For specimens collected from female patients, please indicate whether the patient is pregnant or not.

Methods and Interpretation of Results:

As ZIKV is a flavivirus, serologic tests may detect antibodies to other flaviviruses such as dengue, West Nile, and Yellow Fever (including vaccine recipients). Confirmation of ZIKV, therefore, requires detection of viral RNA by RT-PCR or confirmatory PRNT serologic testing, which is laborious and time-consuming. Confirmatory testing requires neutralizing antibody production, which may appear later than IgM. Patients whose serum samples are IgM and/or IgG positive and are also shown to contain ZIKV-specific antibodies by a PRNT assay are confirmed cases of viral infection. It should be noted that individuals previously infected with or vaccinated against flaviviruses may exhibit cross reactivity in PRNT tests as well, and the test results may be difficult to interpret. Since dengue virus and ZIKV are transmitted by the same types of mosquitoes, co-infections with these viruses are possible. As noted above, if antibody is present against both of these viruses or other related flaviviruses, it may be difficult to determine the virus responsible for current versus past infections.

Turnaround Time:

15 calendar days.

Contact:
Phone: (204) 789-6097 or (204) 789-2106
Fax: (204) 789-2082
Fact Sheets: