Ricin Antigen Capture ELISA

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Detection of ricin toxin in biological samples by ELISA.

Test Category:
Serology
Pathogen:
Ricinus communis
Illnesses and Diseases:
  • Ricin poisoning
Specimen:

Whole blood – minimum 0.5 mL, powder, liquid, beans, plant material, food sample.

Collection Method:

Whole blood – EDTA tube; powder/liquid – submit in a sterile 2 mL o-ring microcentrifuge tube; food/beans/plant material – submit in an appropriately sized container with an o-ring.

Specimen Processing, Storage and Shipping:

Store samples refrigerated until shipped for testing. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Confirmed ricin sample. 

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history.  Type of specimen and date collected. 

Comments:

N/A

Methods and Interpretation of Results:

Ricin Antigen Capture ELISA is performed on samples suspected to contain purified ricin toxin or samples confirmed as positive by real-time PCR.

Turnaround Time:

4 calendar days for a preliminary result and 14 calendar days for a final result.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Maddaloni, M., Cooke, C., Wilkinson, R., Stout, A.V., Eng, L., Pincus, S.H. 2004. Immunological characteristics associated with the protective efficacy of antibodies to ricin. J Immunol 172, 6221-6228.
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