Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) and Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Multi-faceted approach to the molecular typing of Brucella strains.

MLVA Test:  Use of PCR and subsequent fragment size analysis of 11 loci to determine strain relatedness.

SNP Test: Use of seven species specific single-nucleotide polymorphisms (SNPs) to differentiate between Brucella strains using real-time PCR.

Test Category:
Molecular Differentiation
Pathogen:
Brucella spp.
Illnesses and Diseases:
  • Brucellosis
Specimen:

Pure bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Confirmed Brucella spp. infection.

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history.  Type of specimen and date collected.  If possible, include the antibiotics administered and travel history. 

Comments:

N/A

Methods and Interpretation of Results:

MLVA Test: The MLVA pattern will be determined through PCR and sizing of resulting fragments through capillary electrophoresis.   Any matches with previous isolates in the existing databank will be communicated to the client.

SNP Test:  The species specific SNP profile will be determined through real-time PCR and compared to control strains.  

Turnaround Time:

14 calendar days for a final result.

Contact:
Phone: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
References:
  1. Foster, Foster, J.T., et al, 2008. Real-Time PCR Assays of Single-Nucleotide Polymorphisms Defining the Major Brucella Clades
  2. P. Le Flèche, I. Jacques, M. Grayon, et al. 2006. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiology 2006, 6:9.
Related Information: