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Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)

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Accredited by the Standards Council of Canada to Laboratory no. 594 (ISO/IEC 17025)

Requisition Forms

Reference Details


Multi-faceted approach to the molecular typing of classical brucellosis causing Brucella strains.

WGS Test: Molecular typing of Brucella strains using Whole Genome Sequencing data.

SNP Test: Use of seven species specific canonical single-nucleotide polymorphisms to differentiate between Brucella strains using real-time PCR.


Test Category:
Molecular Differentiation
Brucella spp.
Illnesses and Diseases:
  • Brucellosis

Pure bacterial culture.

Collection Method:

Pure bacterial culture – plate or swab.

Specimen Processing, Storage and Shipping:

Store bacterial cultures refrigerated until shipped for testing. 

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.


Patient Criteria:

Confirmed brucellosis causing Brucella spp. infection. 

Accompanying Documentation:

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number.  Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history.  Type of specimen and date collected.  If possible, include the antibiotics administered and travel history. 



Methods and Interpretation of Results:

WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.

SNP Test: The species specific SNP profile is determined through real-time PCR and compared to control strains.  

Turnaround Time:

14 calendar days for a final result.

Phone #: (204) 784-5928 or 1-877-212-6108
Fax: (204) 789-5009
  1. Foster, JT, et al, 2008. Real-Time PCR Assays of Single-Nucleotide Polymorphisms Defining the Major Brucella Clades. J Clin Microbiol. Jan;46(1):296-301.
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