Whole Genome Sequencing (WGS) and Single Nucleotide Polymorphisms (SNPs)
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Requisition Forms
Reference Details
Multi-faceted approach to the molecular typing of classical brucellosis causing Brucella strains.
WGS Test: Molecular typing of Brucella strains using Whole Genome Sequencing data.
SNP Test: Use of seven species specific canonical single-nucleotide polymorphisms to differentiate between Brucella strains using real-time PCR.
- Brucellosis
Pure bacterial culture.
Pure bacterial culture – plate or swab.
Store bacterial cultures refrigerated until shipped for testing.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Confirmed brucellosis causing Brucella spp. infection.
Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.
N/A
WGS Test: The phylogenetic relationships and subtype strains are determined using Whole Genome Sequencing data based on single base pair variants (SNVs) at specific genomic locations.
SNP Test: The species specific SNP profile is determined through real-time PCR and compared to control strains.
14 calendar days for a final result.
- Foster, JT, et al, 2008. Real-Time PCR Assays of Single-Nucleotide Polymorphisms Defining the Major Brucella Clades. J Clin Microbiol. Jan;46(1):296-301.