Prion Diseases Section

Laboratory Details

Division:
Zoonotics and Special Pathogens
Address:
1015 Arlington Street
Winnipeg, Manitoba
R3E 3R3
Phone:
(204) 789-6078
Fax:
(204) 789-5009
Description:

The Prion Diseases Section provides national reference services in support of health care professionals dealing with suspected cases of Creutzfeldt-Jakob Disease (CJD).  Accredited CJD testing includes the detection of increased amounts of protein markers of disease found in patients’ cerebrospinal fluid (CSF).  The levels of two indirect markers of disease, gamma 14-3-3 and total tau are measured by enzyme-linked immunosorbent assay (ELISA) based tests The presence of the pathogenic prion protein in CSF is detected  by the quaking-induced conversion (QuIC) assay.   These CSF based tests are offered as a panel.  Collectively the test results support diagnoses of CJD.

The Prion Diseases Section supports further investigation of CJD cases by providing sequence analysis of PRNP (the gene encoding the prion protein). Sequence analyses confirm or exclude the presence of mutations linked to inherited prion diseases.  Due to the privacy considerations surrounding sequencing of patient DNA, consent must be obtained prior to this analysis being performed.  Consent forms are available through the CJD Surveillance System: 1-888-489-2999. 

Proficiency Panels

Proficiency Panel Reports

Requisition Form

Reference Diagnostic Tests

Reference Diagnostic Tests
Pathogen Test Category Reference Diagnostic Test
Creutzfeldt-Jakob Disease (CJD) - sporadic, genetic and variant forms Host Marker CSF Protein Panel
Disease-associated, misfolded isoform (PrPd) of the host prion protein (PrP) Host Marker PRNP Gene Sequencing