Detection of IgM Antibodies Directed Towards Ross River Virus by ELISA<<Return to Laboratory
Serological detection of IgM antibodies directed towards Ross River virus (RRV) by PanBio IgM ELISA.
- Ross River fever
Serum. Minimum volume of 250 µl required.
2 mL screw cap tubes.
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
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Suspected Ross River virus infection and relevant travel history.
Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, clinical and travel history, and dates of travel of the patient.
All RRV diagnostic samples are tested by both IgM and IgG ELISA to increase the clinical sensitivity.
Detection of anti-RRV IgG antibody in a single serum sample is indicative of past or present exposure to this agent. The presence of RRV specific IgM in a single serum sample is consistent with an acute infection to this agent and meets the criteria for a "probable case". However, an IgG or IgM seroconversion in paired sera is required to document a "confirmed case" of infection with associated illness.
There is increasing evidence for IgM persistence in blood/serum for up to a year or more after arbovirus (i.e. members of the flavivirus, alphavirus, and bunyavirus arthropod borne virus groups) exposure. Thus, detection of IgM by itself may not always be a confirmation of acute infection.
Isolation of an arbovirus, detection of specific antibody by plaque reduction neutralization assay or detection of nucleic acid by Real Time RT-PCR in a clinical specimen would constitute firm evidence of viral association with illness and provide "confirmed case" status.
14 Calendar days.
- Inverness Medical Innovations Australia Pty Ltd. 2008. Ross River Virus IgM ELISA kit instructions.