Pulsed Field Gel Electrophoresis (PFGE)

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Requisition Forms

Reference Details

Description:

Pulsed field gel electrophoresis (PFGE) of Neisseria meningitidis.

Test Category:
Genetic Fingerprinting
Pathogen:
Neisseria meningitidis
Illnesses and Diseases:
  • Meningitis
Specimen:

Pure culture.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send as growthon culture medium (preferred) or on a swab in transport medium. Cultures can be also shipped frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Suspected of invasive disease caused by Neisseria meningitidis.

Accompanying Documentation:

Completed Vaccine Preventable Bacterial Diseases requisition form including sender lab name, address and telephone number. Patient identifier (specimen reference #), date of birth and sex. Type of specimen, date collected and test requested. Vaccine history (if known).

Comments:

N/A

Methods and Interpretation of Results:

See references below.

Turnaround Time:

20 calendar days (unless specified urgent).

Contact:
Phone: (204) 789-2130 or (204) 789-6017
Fax: (204) 789-2018
References:
  1. Chu G, Vollrath D, Davis RW. 1986. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582-5.
  2. Chu G. 1991. Bag model for DNA migration during pulsed-field electrophoresis. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11071-5.
  3. Chu G., "Pulsed-Field Gel Electrophoresis: Theory and Practice," Methods: A Comparison to Methods in Enzymology, vol. 1, No. 2 (1990), pp. 129-142.
  4. SM Clark, E Lai, BW Birren, and L Hood. 1988. A novel instrument for separating large DNA molecules with pulsed homogeneous electric fields. Science. 1988 Sep 2;241(4870):1203-5.