16S rRNA and Mip Gene Sequencing

<<Return to Laboratory

*Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

16S rRNA gene PCR*, sequencing, and phylogenetic analysis. Mip gene sequencing and phylogenetic analysis is used complementarily with 16S gene sequencing and is dependent on the 16S gene sequencing results.

Test Category:
Sequencing
Pathogen:
Legionella spp.
Illnesses and Diseases:
  • Legionellosis
Specimen:

Pure cultures of isolate implicated in clinical illness or environmental isolates.

Collection Method:

Slants or plates of any suitable media and swabs in transport media are all acceptable.

Specimen Processing, Storage and Shipping:

Store and ship samples at room temperature.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Clinical illness with symptoms suggestive of bacterial infection.

Accompanying Documentation:

Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information.  If possible, attach lab results that have already been done at local or provincial laboratories.

Comments:

All patient and strain history must be included. Please note that multiple samples isolated from the same source and patient, and identified as the same organism by the sender, will need an explanation in order for testing to be done on all the samples. Acceptance and/or rejection of the additional samples is at the discretion of the Special Bacteriology lab.   Contact lab for further information.  Please refer to the Guide to Services for test services offered by the NML.

Methods and Interpretation of Results:

16S and mip gene (if applicable) sequencing results are compared against publically-available databases (GenBank) and known type strains.

Turnaround Time:

15 calendar days for the 16S/secondary gene target final report. Report may be delayed in cases where staff or resources are limited, or for poor or slow growing organisms.  In cases where further testing is required, the final report may be delayed and status of request will be forwarded in a preliminary report. Final report is sent once all testing is complete.

Contact:
Phone: (204) 789-2137
Fax: (204) 784-7509
References:
  1. Clinical and Laboratory Standards Institute.  2018. Interpretive Criteria for Identification of bacteria and Fungi by DNA Target Sequencing; Approved Guideline MM18-Ed2. Volume 38, Number 15. Wayne, PA.
  2. Ratcliff RM, Lanser JA, Manning PA, Heuzenroeder MW.  1998.  Sequence-based classification scheme for the genus Legionella targeting the mip gene.  J Clin Microbiol. 36(6):1560-7.
Fact Sheets:
Related Information: