Molecular Detection by Quantitative PCR

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Requisition Forms

Reference Details

Description:

Quantitative PCR detection of Human Herpesvirus 6 (HHV-6) DNA.

Test Category:
Molecular Quantification
Pathogen:
Human Herpesvirus-6 (HHV-6)
Illnesses and Diseases:
  • Roseola
Specimen:

Plasma from immunocompromised patients – 1mL required (minimum 500  uL). CSF from encephalitis cases – 600uL required (minimum 300uL). Minimum volume will not allow repeat testing of samples with equivocal results.

Collection Method:

Collect blood in plasma preparation tube (PPT) or K2EDTA tube (plastic; lavender or pink top). Do not use heparin as an anti-coagulant. Centrifuge tube at room temperature at 1,100 RCF for 10-15 min. Aliquot plasma into separate tube. Collect CSF in a dry tube subject to classic conditions for carrying out lumbar punctures.

Specimen Processing, Storage and Shipping:

Specimens may be stored and shipped refrigerated to NML within 48h of sample collection or must be stored and shipped frozen.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

Patient Criteria:

Specimens are accepted from immunocompromised patients with primary infection or reactivation or patients with encephalitis.
** Please note that specimens for routine monitoring of HHV-6 infection in transplant patients will not be accepted.

Accompanying Documentation:

Completed "Viral STI, Polyoma and Herpesvirus Testing" requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age and sex, specimen reference #, type of specimen, date collected, test requested, reason for testing and any other relevant clinical information.

Comments:

If investigation of HHV-6 chromosomal integration is required, please contact lab for further details prior to specimen collection.

Methods and Interpretation of Results:

DNA is extracted from specimens and tested by real-time PCR using the PrimerDesign Quantification of HHV-6 genesig kit (1), which targets the BHLF3 putative dUTPAse gene. The results are reported as copy number of HHV-6 DNA per mL of original specimen. This assay is for research purposes only.

Turnaround Time:

14 calendar days. STAT samples may be considered under special circumstances. Please contact lab to inquire about shorter turn around time.

Contact:
Phone: (204) 789-6024
Fax: (204) 318-2222
References:
  1. PrimerDesign Quantification of Human Herpes 6 genomes genesig Advanced kit handbook HB10.03.04
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Guidelines:
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