Detection of IgG Antibodies Directed Towards Dengue Virus by ELISA

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Reference Details

Description:

Serological detection of IgG antibodies directed towards Dengue virus by Euroimmun Anti-Dengue Virus ELISA (IgG).

Test Category:
Serology
Pathogen:
Dengue virus
Laboratory:
Illnesses and Diseases:
  • Dengue hemorrhagic fever (DHF)
  • Dengue fever
  • Dengue shock syndrome
Specimen:

Serum. Minimum volume of 250 µl required.

Collection Method:

2 mL screw cap tubes.

Specimen Processing, Storage and Shipping:

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

For additional guidance on the transport of infectious substances in other languages, please click on the link below.

http://www.who.int/ihr/capacity-strengthening/infectious-substances/en/

 

Patient Criteria:

Suspected dengue virus infection and relevant travel history.

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, clinical symptoms, and travel history of patient.

Comments:

All diagnostic samples are tested by both IgM and IgG ELISA to increase the clinical sensitivity.

Methods and Interpretation of Results:

Due to the cross-reactive nature of flavivirus antibody, the detection of anti-flavivirus IgG (Eg. Zika, Powassan etc.) in a single serum sample is indicative of past or present exposure to this agent, or a related agent from the same virus genus. The presence of DEN specific IgM in a single serum sample is consistent with an acute infection to this agent (or a related flavivirus, note that flavivirus IgM serological procedures are more specific than IgG serology for this genera of arboviruses) and meets the criteria for a "probable case". However, a 4-fold rise or greater in neutralizing antibody titre, or an IgG or IgM seroconversion in paired sera, is required to document a "confirmed case" of infection with associated illness.

There is increasing evidence for IgM persistence in blood/serum for up to a year or more after arbovirus (Eg. members of the flavivirus, alphavirus, and bunyavirus arthropod borne virus groups) exposure.  Thus, detection of IgM by itself may not always be a confirmation of acute infection.

Isolation of an arbovirus, detection of specific antibody by plaque reduction neutralization assay or detection of nucleic acid by real-time RT-PCR in a clinical specimen would constitute firm evidence of viral association with illness and provide "confirmed case" status.

Turnaround Time:

14 calendar days.

Contact:
Phone: (204) 789-6097 or (204) 789-2106
Fax: (204) 789-2082
References:
  1. Euroimmun Medizinische Labordiagnostika AG. Anti-Dengue Virus ELISA (IgG) test Instructions Version 05/03/2019.
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